Sample preparation is easy: fill each of the three wells with a gel (e.g., collagen, Matrigel, or fibrinogen). Then, simply seed the cells into the channel, where they will adhere to the gel. For applying defined shear stress, the slide is connected to a pump (e.g., to the ibidi Pump System). Thanks to the ibidi Polymer Coverslip on the slide’s top and bottom, the cells can be imaged using high-resolution microscopy.
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