GMO-free feed for appropriately declared milk? Horse meat in a lasagna? Cod instead of pike-perch fillet? Celery in parsley? For many years now, lifeprint GmbH has been using various molecular biological methods to analyse the genetic material DNA in order to detect both contaminations and adulterations in food and animal feed.
Now, a completely new method in food and feed analysis offers far more possibilities for the analysis of products and raw materials: Next Generation Sequencing (NGS).
While PCR is limited to the targeted search for one or more previously defined species and sequencing is essentially restricted to monoproducts, Next Generation Sequencing also provides information in mixed products about the species whose DNA is contained in the sample. Two methods are available at lifeprint GmbH: one method for the identification of vertebrate species and another method for the identification of land plant species.
How does the NGS work? With this analysis, millions of sequences can be determined simultaneously from different samples. NGS generates huge amounts of data on sequences for each sample, which are then evaluated using bioinformatic methods. For example, it is not only possible to determine whether Origanum was stretched with olive leaves, but also whether one or more other unexpected further species are present in Origanum.
Also with animal products wrongly declared products are found again and again, with which expensive species are partly or completely substituted by cheaper ones1.
For both plant and animal products, Next Generation Sequencing is a very good way of detecting cases of adulteration. The method is primarily qualitative, but the number of different sequences obtained can be included for evaluation.
The two Next Generation Sequencing methods validated by lifeprint GmbH in 2018 have been available as accredited methods since July 2019.
Collaborating with the Tentamus Center for Food Fraud (TCF2), lifeprint GmbH can also offer its customers access to a wide range of state-of-the-art analytical methods for the detection of food fraud.
1https://trello.com/...
Now, a completely new method in food and feed analysis offers far more possibilities for the analysis of products and raw materials: Next Generation Sequencing (NGS).
While PCR is limited to the targeted search for one or more previously defined species and sequencing is essentially restricted to monoproducts, Next Generation Sequencing also provides information in mixed products about the species whose DNA is contained in the sample. Two methods are available at lifeprint GmbH: one method for the identification of vertebrate species and another method for the identification of land plant species.
How does the NGS work? With this analysis, millions of sequences can be determined simultaneously from different samples. NGS generates huge amounts of data on sequences for each sample, which are then evaluated using bioinformatic methods. For example, it is not only possible to determine whether Origanum was stretched with olive leaves, but also whether one or more other unexpected further species are present in Origanum.
Also with animal products wrongly declared products are found again and again, with which expensive species are partly or completely substituted by cheaper ones1.
For both plant and animal products, Next Generation Sequencing is a very good way of detecting cases of adulteration. The method is primarily qualitative, but the number of different sequences obtained can be included for evaluation.
The two Next Generation Sequencing methods validated by lifeprint GmbH in 2018 have been available as accredited methods since July 2019.
Collaborating with the Tentamus Center for Food Fraud (TCF2), lifeprint GmbH can also offer its customers access to a wide range of state-of-the-art analytical methods for the detection of food fraud.
1https://trello.com/...
